Cyanate Degradation in Different Matrices Using Heat-Purified Enzymes

A green and low-cost removal method for cyanate, a toxic byproduct from the treatment of cyanide, is still needed. Cyanase converts cyanate to CO2 NH3, but its industrial practicality limited because reaction requires HCO3− as substrate. In this study, we used carbonic anhydrase Sulfurihydrogenibium azorense (SazCA) provide cyanase Thermomyces lanuginosus (TlCyn); both TlCyn SazCA were purified by one-step heating without prior cell lysis. The heat resulted in higher activities enzymes than conventional two-step process. From 50 mL-culture, highest total activity 147 U 47,174 WAU was obtained 5 min at 60 80 °C SazCA, respectively. coupled enzymatic system degrade three different matrices: mM Tris-HCl (pH 8), wastewater, artificial wastewater. with addition 0.75 (Wilbur-Anderson unit) degradation using 0.5 NaHCO3 similar that 3 NaHCO3, indicating an 83% reduction NaHCO3. We have demonstrated dependence on can be effectively alleviated heat-purified SazCA; therefore improved.